Role of emodin and somatostatin in protection of intestinal mucosal barrier cells in rats with acute pancreatitis

Objective: To explore the alterative mechanism of intestinal mucosal cell membrane fluidity and calcium pump in rats with acute pancreatitis(AP),and to study the effects of emodin and somatostatin on them.Methods: Eighty male SD rats were randomly divided into four groups:sham operation group(SO),normal saline(NS) group,emodin group and somatostatin group.Intestinal mucosal cell membrane Ca²⁺-Mg²⁺ATPase activity,fluorescence polarization,malondialdehyde(MDA) content and blood flow of intestinal mucosa were determined 6 hours after the model rats of acute pancreatitis were established.Results: In the emodin group and somatostatin group,the MDA content was lower than that in the SO group(P<0.01),Ca²⁺-Mg²⁺ATPase activity and membrane fluidity were higher than those in SO group(P<0.01)and the decreased value of blood flow of intestinal mucosa was lower compared with that of SO group.Conclusions: Emodin and somatostatin can protect the function of intestinal mucosal barrier by ameliorating intestinal mucosa ischemia,improving calcium pump activity and elevating intestinal mucosal cell membrane fluidity.