A study on interference of N-Ras by RNA to enhance radiosensitivity of hepatoma carcinoma cell HepG2

Objective:To investigate the radiosensitivity of silencing N-Ras by RNA interference in Hepatoma carcinoma cell HepG2.Methods:N-Ras RNA interference (RNAi) vector was constructed by using pcDNA 6.2-GW/EmGFP-miR plasmid.The RNAi effect was detected by RT-PCR,Western blot,immunohistochemisty and MTT method.Survival curves for each cell line were obtained by measuring the colony forming abilities of irradiated cell populations.Results:After silencing the N-Ras by RNAi,the expression of N-Ras mRNA,N-Ras protein and the survival of hepatoma carcinoma cell HepG2 line were significant difference in statistics.Conclusions:RNAi targeting silenced N-Ras may increase the radiosensitivity of hepatoma carcinoma cell HepG2 line.