Optimization of culture condition for human epidermis melanocytes growth in vitro

Objective: To investigate the effects of two mediums supplemented with various ingredient on epidermal melanocytes culture.Methods: The foreskins from an infant were treated with dispase II to separate epidermis from dermis,and treated with trypsin for obtaining single cell suspensions.The cells were cultured in different media supplemented with cell growth component.The proliferation and dendrites of cultured melanocytes were observed day by day.Results: The melanocytes from infant foreskin showed potent vitality.The fibroblasts cells in culture decreased after dispase and trypsin digesting specimen.The rapid proliferation and increased dendrites of melanocytes in 254 media supplemented with human melanocyte growth supplement(HMGS) were observed.Conclusions: Infant foreskins treated with dispase and trypsin and cultured with 254 containing HMGS,were suitable for melanocyte culture.