Comparison of endotoxin content between Gram negative bacteria and their L-forms

Objective: To compare endotoxin content between Gram negative bacteria and their L-forms.Methods: The endotoxin content was detected by qualitative and quantitative limulus tests in E.coli and P.aeruginosa and their stable L-forms.Results: The qualitative limulus tests of Gram negative bacterial L-forms were positive.The endotoxin content of E.coli L-forms is 25.1% of their parental bacteria,and P.aeruginosa L-forms was 36.4% of parental.Conclusions: Gram negative bacterial L-forms generate less endotoxin.