The expressions of Peroxiredoxin 6,serine protease inhibitor B1 and translationally controlled tumor protein protein in neutrophils of Acinetobacter baumannii sepsis rats

Objective: To explore the expressions of the Peroxiredoxin 6(PRDX6),serine protease inhibitor B1(SERPIN B1) and translation control tumor protein translationally controlled tumor protein(TCTP) in neutrophils of Acinetobacter baumannii sepsis rats using Western blot technology at different points time.Methods: The Acinetobacter baumannii rat model was established by intraperitoneal injection with Acinetobacter baumannii.The total proteins of neutrophils in normal rats and rats after 6 and 12 h of sepsis were harvested.The protein levels of PRDX6 SERPIN B1 and TCTP in normal and sepsis rats were detected by western blot,and compared with the results of two-dimensional gel electrophoresis.Results: The PRDX6 expression levels in neutrophils of Acinetobacter baumannii rats after 6 and 12 h of sepsis were significantly lower than that of normal rats(P< 0.01),the increasing degree of which in rats after 6 h of sepsis was more significantly than that in rats after 12 h of sepsis(P< 0.01).The SERPIN B1 and TCTP expression levels in neutrophils of Acinetobacter baumannii rats after 6 and 12 h of sepsis were significantly higher than those of normal rats(P< 0.01),the differences of which between sepsis rats after 6 and 12 h of sepsis were not statistically significant(P >0.05).The expression levels of PRDX6 SERPIN B1 and TCTP were in accordance with the results of proteomics.Conclusions: The expressions of PRDX6、SERPIN B1 and TCTP in neutrophils of Acinetobacter baumannii sepsis rats are different from the normal rats,which can used as the target molecules in the early diagnosis and treatment of Acinetobacter baumannii.