The survivin expression in brain glioma tissues and effection of survivin antisense oligodexynucleotides on U251 cells

Objective: To observe the expression of survivin in brain glioma tissues and effects of survivin antisense oligodexynucleodides(ASODN) on the proliferation and apoptosis of U251 cells.Methods: RT-PCR method was performed for detecting survivin mRNA expression in specimens.The glioma cells U251 transferred with survivin ASODN for 48 h,the proliferation and apoptosis were measured by flow cytometry(FCM) and cell morphology.Results: Survivin mRNA was expressed in brain glioma tissues,and the positive rate was 76.7%.When U251 cells were treated with different concentration of ASODN at 100 nmol/L,300 nmol/L,and 500 nmol/L,the apotosis rates were (19.46±3.85)%,(38.60±1.85)%,and(55.34±3.16)%,respectively;and the proliferation index(PI) were(52.24±6.59)%,(36.14±4.01)%,and(24.14±5.57)%,respectively,the difference was distinct compared with control and groups of different concentration(P<0.05-P<0.01).Conclusions: Survivin mRNA was expressed in the brain glioma tissues,survivin ASODN efficiently inhibits the proliferation and induce apotosis of U251 cells in a dose-dependent manner.