Molecular cloning and sequence analysis genes of human interleukin-23 p19 subunit

Objective:To clone genes for encoding human interleukin-23 (IL-23) p19 subunit.Methods:Peripheral blood mononuclear cells (PBMCs) of human were isolated and cultured for 10 hours,and then stimulated with 100 ng/ml LPS for 12 hours.Total RNA from these stimulated PBMCs were extracted,and cDNA of genes for human IL-23 p19 subunit were ampliphied by RT-PCR assay and inserted into pMD18-T vector.Results:The PCR production of human IL-23 p19 was verified by Electrophoresis proved to be 734 bp,result of sequencing was the same as the gene sequence of GenBank.Conclusions:cDNA encoding human interleukin-23 p19 was successfully cloned into cloning vector pMD18-T.