Preparation of rabbit sera of anti-Taenia solium onchosphere and in vivo expression of recombinant plasmid pcDNA3.1/TSO45-4B in skeletal muscle of vaccinated mice

Objective: To prepare anti-onchosphere rabbit sera and observe the in vivo expression of recombinant plasmid pcDNA3.1/TSO45-4B containing gene of stage-specific antigen TSO45-4B of Taenia solium onchosphere.Methods: The soluble antigen of onchosphere collected from hatched eggs was injected into rabbits,and the anti-onchosphere rabbit sera were collected and detected by Dot-Blot and ELISA.The recombinant plasmid pcDNA3.1/TSO45-4B was injected into Kunming mice's skeletal muscle and its expression was detected by immunohistochemistry assay.Results: The titer of polyclonal antibody to antigen of onchosphere in rabbit serum was(1:20 100).The specific masculine brown-yellow color reaction was seen in the muscle fibers of recombinant plasmid injected group.Conclusions: The successful expression of pcDNA3.1/TSO45-4B has provided the basis of in vivo expression for developing DNA vaccine for prevention of cysticercosis.