Synergic effect of the combination of thermotherapy and 10-hydroxycamp-tothecine(HCPT) on hepatoma cells in vitro

Objective: To investigate whether the combination of thermotherapy and HCPT has a synergic effect on the hepatoma cells(SMMC-7721 cell line) in vitro.Methods: The experiment consists of four groups:thermotherapy,chemotherapy(HCPT),thermo-chemotherapy(chermotherapy combined with HCPT) and control group.Results: Thermotherapy within 30 minutes with a temperature of 43℃,44.5℃ had an obviously inhibited SMMC-7721 cells(P<0.01),while 40℃,41.5℃ hardly had any effect on the cells(P>0.05).HCPT had a great cytotoxicity effect on SMMC-7721 cells in dose and time-dependant manner(P<0.01).The cytotoxicity could be enhanced when HCPT was combined with thermotherapy(P<0.01).Conclusions: The combination of HCPT and themotherapy has a remarkable synergic effect on hepatoma cells.