Detection of Staphylococcus aureus toxigenes and its clinical application

Objective: To study genic feature of Staphylococcal enterotoxins(SEs),exfoliative toxins(ETs),toxic shock syndrome toxin 1(TSST-1) and Panton-Valentine leukociclin(PVL) carried by both hospital-acquired Staphylococcus aureus(HA-SA) and community-acquired Staphylococcus aureus(CA-SA).Methods: The genes of SEA-SEE,ETA,ETB,TSST-1 and PVL were detected by PCR.Results: Out of 116 SA strains,85 strains were HA-SA.SEA was detected in 6 strains,SEC in 1 strain,SED in 1 strain,and PVL in 2 strains.Thirty one strains were CA-SA.SEA was detected in 1 strain,SEB in 1 strain,SED in 1 strain,and PVL in 7 strains.SEE,ETA,ETB and TSST-1 were negative in all SA.PVL positive rate detected in CA-SA was much higher than that in HA-SA(P<0.005),while positive rates of SEs,ETs,TSST-1 were not significant different between CA-SA and HA-SA(P>0.05).Conclusions: Staphylococcus aureus may secrete various toxins,detecting should pay attention to whose toxins while separating Staphylococcus aureus.