The effect of Ara-C at low concentrations on CD147 expression in Jurkat cells

Objective: To investigate the effect of Ara-C at low concentrations on extracellular matrix metalloproteinase inducer (CD147) expression in Jurkat cells. Methods: Jurkat cells were divided into six groups:control group and cells treated with different concentrations of Ara-C at 1,5,10,20,and 30 ng/ml. CD147 mRNA and protein expression were assayed by RT-PCR,Western blot and flow cytometry,respectively. The colony formation in Jurkat cells which was stimulated with Ara-C or not was observed by inverted microscope. Results: Different concentrations of Ara-C significantly up-regulated the expressions of CD147 mRNA and protein,and increased the colony formation in Jurkat cells (P < 0.01). Conclusions: Ara-C at low concentrations can enhance the expression of CD147 and increase the adhesive force in Jurkat cells.