Construction and identification of siRNA recombinant plasmid of DNMTl and DNMT3b genes

Objective:To construct and identificate the siRNA recombinant plasmid of DNMT1 and DNMT3b genes.Methods:Four types of siRNA recombinant plasmid of DNMT1 or DNMT3b gene were constructed,and identificated.Results:After digested withBamH Ⅰ and pstⅠ enzymes,all plasmids showed positive for recombinant vector plasmids.Two clones of each type of vector plasimid were selected for,sequencing identification.The results of sequencing identification were consistent with the inserted oligonucleotides.Conclusions:The siRNA recombinant plasmids of DNMT1 and DNMT3b genes were constructed corectly and could be used for further study.