Association of metastasis suppressor gene KAI1 expression and lymphangiogenesis with non-small cell lung cancer and its clinical significance

Objective:To study the synergy between down-regulation or lack of KAI1(one of metastasis suppressor genes) and metastasis of lymph node in non-small cell lung carcinoma(NSCLC).Methods:The expression of KAI1 and Podoplanin which was used to show lymphatic vessel density(LVD) were detected in 68 paraffin sections of NSCLC with immunohistochemistry(EliVision methods),and logistic regression analysis was used to show the relationship between the expression and metastasis of lymph nodes.Results:The positive rates of KAI1 in group one(with metastasis of lymph node) and group two(without metastasis of lymph node) were 30.3% and 88.6%(P<0.01),respectively.The difference of LVD was significant between group one(15.73±0.74) and group two (8.31±0.32)(P<0.01).Logistic regression analysis showed that LVD had an impact on metastasis of lymph node(P<0.05);the odds risk(OR) was 2.065 and the confidence interval of 95% was (1.202,3.547).The down-regulation of KAI1 had no statistical significance(P=0.375),and the confidence interval of 95% was(0.003,8.714).There was no significant synergy between LVD and the expression of KAI1 in metastasis of lymph node(P>0.05).Conclusions:There exists no obvious synergies between down-regulation of KAI1 and lymphangiogenesis in metastasis of lymph node in non-small cell lung carcinoma.