The experimental study of the transfection recombinant lentiviral vector with FasL gene into rat kidney

Objective:To explore the target gene expression of SD rat kidney transfected recombinant lentiviral vector with FasL and provide a basis of transplantation study.Methods:The recombinant lentiviral vector and empty vector(control) were injected into SD rat kidney through renal artery.The organization form and gray value of kidney tissues were detected by HE and immunohistochemistry(IHC) at days 3,7,15,25 and 40 after transfection and statistic analyzed.Results:No morphologic changes and inflammatory cells infiltration were found in kidney by HE staining..The peak of FasL gene expression was on the 15 day,which had significant difference compared with the control group(P0.05).Conclusions:The recombinant lentiviral vector with FasL gene can be successfully transfected into SD rat kidney,which can express target gene.