Detection of resistance gene of carbapenem-resistant Klebsiella pneumoniae from intensive care units

Objective:To investigate the molecular epidemiology and mechanism of carbapenem resistance of Klebsiella pneumonia from intensive care units(ICU). Methods:Thirteen strains of Klebsiella pneumonia with carbapenem resistance were isolated from ICU. The minimum inhibitory concentrations(MIC) were detected by using agar-dilution, polymerase chain reaction(PCR) and cloning and sequencing of resistance genes were used to analyzed the resistant mechanism. Enterobacterial repetitive intergenic consensus-PCR was performed to analyze the molecular epidemiology. Results:The MICs of imipenem of 13 strains of Klebsiella pneumoniae were from 16 to 1 024 g/ml, which were blaKPC-2 and blaSHV using the PCR product cloning and sequencing method. Conclusions: Carbapenemase blaKPC-2 is the major factor of carbapenem resistance, and lactamase blaSHV participates multiple mechanisms of resistance.