Multiplex polymerase chain reaction for detection of drug resistance genes of mycobacterium tuberculosis

Objective:To establish a rapid detection method of mycobacterium tuberculosis. Methods:Multiplex polymerase chain reaction(PCR) was employed to dectect katG,IS1081,inhA and rpoB genes of mycobacterium tuberculosis. Forty-eight DNA samples were obtained from the national institute for the control of pharmaceutical and biological products (NICPBP), forty drug resistant mycobacterium tuberculosis were isolated and 40 drug sensitive mycobacterium tuberculosis were isolated from the culture-based bacterias and 46 from clinical samples. All results have been confirmed by sequencing. Results:These genes were successfully 1detected in all samples by multiplex PCR. Conclusions:Multiplex PCR can be used to detect target genes of mycobacterium tuberculosis rapidly.