Effect of arsenic trioxide on telomerase hTERT promoter activity in HL-60 cells

Objective：To investigate the molecular mechanism of arsenic trioxide (As2O3)inhibiting telomerase human telomerasereverse transcriptase(hTERT) expression in HL-60 cells. Methods：The different fragments of hTERT promoter luoiferase geneexpression vector were construc;ted and transfected into HL-60 cells with or without As2O3. The changes of luoiferase activity whichindirectly rellected the role of As2O3 on hTERT promoter different fragments were detected. Results：The luoiferase activities in differentfragments of hTERT promoter gene expression vector were decreased with As2O3(P0.05toP0.01)Conclusions：As2O3 can inhibit telomerase activity of hTERT promoter in HL-60 cells