Construction and identification of a expression vector driven by human telomerase reverse transcriptase gene promotor

Objective: To construc and identify the expression vector which contains a luciferase gene driven by human telomerase reverse transcriptase(hTERT) gene promotor.Methods: The fragment of hTERT promoter about 1 100 bp was acquired by nested PCR amplification,the correct sequence was inserted into the luciferase gene expression vector.In order to get a lot of containing hTERT promoter luciferase recombinant,the construction of pGL3-hTERTp recombinant was transformed into JM190 bacteria.Restriction endonuclease and PCR were used to identify the recombinant and sequencing.Results: After identifying which used restriction endonuclease and PCR method,we successfully constructed the recombinant of hTERT promoter luciferase gene expression vector.Conclusions: A novel recombinant luciferase gene expression vector driven by hTERT promoter is made successfully,and the newly constructed recombinant is useful for study the molecular mechanism that As₂O₃ inhibits the expression of telomerase of HL-60 cells.