Expression of human papillomavirus and Epstein-Barr virus in cervical intraepithelial neoplasia

Objective:To explore the expression of human papillomavirus(HPV) and Epstein-Barr virus(EBV) in cervical intraepithelial neoplasia(CIN).Methods:The high-risk HPV(16/18),low-risk HPV(6/11)and EBV of 130 cases of CIN(50 CIN1,40 CIN2,40 CIN3),10 cases of invasive cervical carcinomas,10 cervical condyloma-like lesions and 10 normal mucosa of the uterine cervix were detected using in situ hybridization(ISH) technique.Results:The positive expression rates of HPV16/18 in CIN1,CIN2 and CIN3 were 44.0%,37.5% and 62.5%,respectively;and positive HPV16/18 was detected in 8/10 of the invasive cervical carcinomas,10/10 of cervical condyloma-like lesions and 3/10 of normal mucosa of the uterine cervix(P0.01).The positive expression rates of HPV6/11 in CIN1,CIN2 and CIN3 were 36.0%,52.5% and 70.0%,respectively;and positive HPV6/11 was detected in 6/10 of the invasive cervical carcinomas,9/10 of cervical condyloma-like lesions and 3/10 of normal mucosa of the uterine cervix(P0.01).The combined positive expression rates of HPV16/18 and HPV6/11 in CIN1,CIN2 and CIN3 were 26.0%,22.5% and 50.0%,respectively;and combined positive expression of HPV16/18 and HPV6/11 was detected in 6/10 of the invasive cervical carcinomas,7/10 of cervical condyloma-like lesions and 2/10 of normal mucosa of the uterine cervix(P0.01).The positive expression rates of EBV in CIN1,CIN2 and CIN3 were 24.0%,12.5% and 22.5%,respectively;and EBV was detected positive in 3/10 of the invasive cervical carcinomas,0/10 of cervical condyloma-like lesions and 0/10 of normal mucosa of the uterine cervix(P0.05).Conclusions:HPV infection is the major etiological factor for CIN,clinically often coinfected with some variants.The signification of positive expression of EBV in CINs and invasive cervical carcinomas is still debatable.