Protective effects of sevoflurane on liver ischemia reperfusion injury in rabbits

Objective:To investigate the effects of sevoflurane on liver ischemia reperfusion injury in rabbits.Methods:Twenty-0ne rabbits were randomly divided into sham operation group(S),ischemia reperfusion group(IR) and sevoflurane ischemia reperfusion group(Sev-IR).Rabbits in IR group and Sev-IR group suffered from ischemia for 45 minutes and were followed by reperfusion for 60 minutes.Sev-IR group was anesthetized with sevoflurane.The concentrations of MDA and XOD of liver tissue were determined.The histopathologic changes in the liver were also observed.The expressions of c-fos protein were observed through immunohistochemisty.Results:The concentrations of MDA and XOD increased significantly after liver ischemia-reperfusion.The sinusoid was expanded and congestion obviously.The hepatic cells were hydropic degeneration and vacuole degenerated.Some of them were spotty necrosis.The protein of c-fos was positive expression.When using sevoflurane,the concentrations of MDA and XOD were statistical significantly decreased compared with IR group(P0.05).The hepatic histopathological changes were improved.The degree of degeneration and necrosis of hepatic cells and the congestion of hepatic sinusoid lessened.The expression of c-fos was down regulated significantly(P0.05).Conclusions:The injury of liver ischemia reperfusion can release mass oxygen radicals and increase the expression of c-fos.This can induce hepatic tissue injury.Sevoflurane can protect liver from ischemia reperfusion injury.Its possible mechanisms are restraining excess oxidizing reaction and decreasing the expression of c-fos protein.