Construction of glial cell line-derived neurotrophic growth factor gene fluorescent eukaryotic cell expression vector

Objective: To construct glial cell line-derived neurotrophic growth factor（GDNF） gene fluorescent eukaryotic expression plasmid.Methods: The coding sequence of GDNF was amplified by PCR,the GDNF gene was cloned into plasmid pEGFP-N₁,and the recombinant vector was selected and identified by restriction enzyme analysis and nucleotide sequence determination.Results: Correct construction of pEGFP-N₁-GDNF was identified by methods of restriction enzyme analysis,and nucleotide sequence determination.Conclusions: An eukaryotic expression plasmid pEGFP-N₁-GDNF has been constructed successfully,which will provide the basis for studying the gene of GDNF.