Analysis of KPC genotype in imipenem resistant-Klebsiella pneumoniae

Objective: To analyze the KPC genotype in imipenem resistant-Klebsiella pneumoniae. Methods: The modified Hodge test (MHT) was used to screen the KPC enzyme-producing Klebsiella pneumoniae; the KPC gene was confirmed by PCR and DNA sequencing, and the KPC genotype was further confirmed by comparison with GenBank from internet. Homology of KPC was analyzed by using pulsed-field gel electrophoresis(PFGE) . Results: Ten of the 13 strains(76. 92%) of imipenem resistant-Klebsiella pneumoniae were proved positive by MHT,2 (15. 38%) of which presented positive KPC gene; and the KPC gene was confirmed as type-2 by comparision with GenBank. The Results of PFGE demonstrated that the 2 strains came from the same clone. Conclusions: KPC-2 type carbapenemases gene in Klebsiella pneumoniae has been isolated.