Effect of high glucose on the expressions of CD83,CD86, CD36,CCR7 and -opioid receptor in dendritic cells

Objective: The aim of the present study was to investigate the expressions of scavenger receptor CD36,CCR7,CD83,CD86 and -opioid receptor( MOR) in dendritic cells( DC) induced by high glucose,then explore the effect of hyperglycemia on atherosclerosis. Methods: Immature dendritic cells( imDC) were extracted from normal human peripheral blood mono-nuclear cells( PBMC) which were isolated and cultured with cytokines including GM-CSF and IL-4. After 7 days,the imDC were separated into 3 group,and differently cultured in RPMI1640 medium with either 5 mmol / L D-glucose( NG group),10 mmol / L glucose( TG group) and 25 mmol / L D-glucose( HG group) for 48 hours,and the expressions of the surface markers( CD83,CD86,CD36 and CCR7) in DC were analyzed by flow cytometry. Results: Compared with NG group,the expressions of CD36,CD83,CD86 and CCR7 were significantly upregulated in TG and HG groups( P 0. 05 to P 0. 01). Compared with NG group,the expression of MOR was significuntly upregulated in HG group( P 0. 05). And compared with TG group,the expressions of CD36,CD83 and CD86 were significantly increased in HG group( P 0. 01). Conclusions: High glucose can promote the expressions of surface molecular CD36,MOR and CCR7 in DC.