Determination of naftopidil preparation by HP LC method

Objective: To establish an HP LC method for determination of naftopidil preparations. Methods: In this study, Waters C18 column(150. 0 mm 4. 6 mm, 5 m) was used, with acetonitrile-methanol-0. 02 mol/L sodium acetate(40∶ 30∶ 30) as mobile phase at a flow rate of 1. 0 ml/min, and the detection wavelength was 283 nm. The injection volume was 10 l and column temperature was set at 30 ℃. Results: The calibration curve was over the range of 0. 998-29. 940 g/ml(with the correlation coefficient of 0. 9999) . The mean recovery of three concentration were 101. 61%, 100. 44% and 99. 28% while the RSD(%) was 0. 25, 0. 12 and 0. 44, respectively. Conclusions: The method is simple, accurate, reproducible and suitable for the quality control of Naftopidil preparations.