The biological characteristics of isolation and culture of retinal stem cell in neonatal rat
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Abstract
Objective:To observe the Results of isolation and culture of retinal stem cell in the neonatal rat and investigate their biological characteristics. Methods:The mechanical enzyme separation in vitro amplification of rat retinal stem cells was done,the capacity of cell proliferation and growth curve of CCK-8 detection were observed;cell differentiation was induced,then the expressions of nestin,bromodeoxyuridine,microtubule-associated protein-2,glial fibrillary acidic protein and thymus glycoprotein-1. 1 in cells were detected by immunocytochemical method. Results:The primary retinal stem cells formed a large number of cells in the ball suspended growth,the passaged cells could also form a ball of cells,and behaved as linear growth,the nestin and bromodeoxyuridine expressions were positive;glial fibrillary acidic protein,microtubule-associated protein-2,thymus glycoprotein-1. 1 were positively expressed in part of cells after induction of differentiation. Conclusions:The neonatal SD rat retinal stem cells can be isolated,cultured,and amplified in vitro,expressed the characteristics of stem cells,it can differentiate into retinal ganglion cells.
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