Effect of magnesium sulfate pretreatment on content of nitrous oxide and activity of nitric oxide synthase following global cerebral ischemic reperfusion injury in mice

Objective: To study the effect of Magnesium sulfate(MgSO₄) pretreatment on the content of nitrics oxide(NO) and the activitiy of nitric oxide synthase(NOS) following global cerebral ischemia reperfusion(I/R) injury in mice.Methods: The mice were randomly divided into sham operation group,I/R group and pretreatment groups(pretreated with low,medium and high doses of MgSO₄).Global cerebral ischemia-reperfusion injury model was made.The change of EEG was monitored and the pathological change in morphology was observed.The content of NO and the activity of NOS and inducible NOS(iNOS) were also detected.Results: The change of ischemic impairment in MgSO₄ pretreatment groups was lighter than that in I/R group.The contents of NO and the activities of NOS and iNOS in the sham operation group and MgSO₄ pretreatment groups were all significantly lower than those of I/R group(P<0.05-P<0.01).Conclusions: MgSO₄ pretreatment may reduce the degree of neuron injury and have protective effect on cerebral ischemia-reperfusion injury.The mechanism may be associated with the decrease of the content of NO as well as the activities of NOS and iNOS.