Glucose decreases apolipoprotein E expression in THP-1 macrophages

Objective: To observe the influence of glucose on the expression of Apolipoprotein E(Apo E) in human monocytic leukemia cell line(THP-1) macrophages.Methods: THP-1 cells were induced to macrophages with phorbol,and then treated with RPMI 1640 culture medium containing diferent concentrations of glucose for 72 hours.Apo E mRNA and protein level were analyzed by RT-PCR and Western Blot respectively,meanwhile the viability of macrophages was assayed with MTT method.Results: Glucose significantly decreased Apo E expression of THP-1 macrophages in a dose-dependent manner(20-30 mmol/L).Conclusions: Glucose decreases Apo E expression of THP-1 macrophages,which might be one of the mechanisms of proatherosclerosis of glucose.