Cloning and expression of Plasmodium vivax merozoite surface protein-1-19 gene

Objective: To construct a vector which clone and express Plasmodium vivax MSP1-19 Gene(Pv MSP1-19). Methods: The Pv MSP1-19 was cloned into the expression vector pET₂8a. The recombinant expression vector was transformed into E. coli,and the Pv MSP1-19 protein was expressed under IPTG induction. The recombinant protein was purified by affinity chromatography and the fusion protein was characterized by SDS-PAGE and Western blot. Results: The Pv MSP1-19 gene in plasmid pET₂8a was expressed in E. coli as a fusion protein. The fusion protein could be reacted specifically with Plasmodium vivax patient serum. Conclusions: The Pv MSP1-19 gene was expressed successfully in E. coli,which provides the necessary basis for preparing vaccine in human.