Effects of ginkgo biloba extraction combined with edaravone on expression of NO and NOS in Qi deficient and blood stasis type of rat brain tissue with cerebral ischemia reperfusion injury

Objective: To observe the effects of ginkgo blades extraction(EGb) combined with edaravone(ED) on expressions of NO and NOS in Qi deficient and blood stasis type of rat brain tissue with cerebral ischemia reperfusion injury. Methods: Rats model with Qi deficient and blood stasis were reproduced by hunger,fatigue and high-fat diet. Then the suture method was used to block up the middle cerebral artery for 2 hours,followed by 72 hours' reperfusion therapy. The expressions of NO and NOS in brain tissue were evaluated on the rats model treated with EGb,ED and EGb combined with ED respectively. Results: Compared with model group,EGb and ED all decreased the levels of NO/iNOS and TNOS in Qi deficient and blood stasis type of rat brain tissues with cerebral ischemia reperfusion injury,and the injuries to neurons(P < 0.01). The effect was significant especially in group of EGb combinied with ED(P < 0.01). Conclusions: EGb associated ED can obviously inhibit the levels of NO in in Qi deficient and blood stasis type of rat brain tissues with cerebral ischemia reperfusion injury,and the mechanism of it may be through reducing the expression of iNOS and TNOS proteins in brain tissue to remove the free radicals,reduce nerve cell damages,and protect brain tissue.