Study on staphylococcal enterotoxin B production and its gene of Staphylococcus aureus L-forms

Objective: To investigate staphylococcal enterotoxin B(SEB) production and its gene of Staphylococcus aureus(S.aureus) L-forms.Methods: The production of SEB was examined by method ELISA and its gene was observed by PCR after S.aureus was induced into stable L-forms.Results: There was no significant difference in SEB production between S.aureus and its L-forms(P>0.05),and positive band of (478 bp) was observed by PCR in S.aureus L-forms.Conclusions: SEB production is not changed and its gene is not lost after S.aureus is changed into L-forms.