The effect of the increasing white blood cells on the hemoglobin level

Objective: To investigate the effects of the increasing of white blood cells(WBC) on the hemoglobin(Hb) level.Methods: The Hb values of 90 blood specimens[WBC value for (10 to <20)×10⁹/L in 30 cases,(20 to <40)×10⁹/L in 30 cases and ≥40×10⁹/L in 30 cases] were determined by XE-2100 blood analyzer and the reference method of hemoglobinometry(HiCN colorimetric method).The turbidity of specimen was measured at the 660 nm wave length of spectrophotometer by HiCN colorimetric method,the WBC in specimens were removed by high-speed centrifuge,and then the Hb values in supernatant were determined by the colorimetric method.The Hb values between before and after centrifuge were compared.Results: The effects of the WBC value≥40×10⁹/L on Hb was great.With the increase of the WBC value,the turbidity was more higher,and the effect of Hb was the more obvious(P<0.05).Conclusions: The high WBC can increase the false Hb value.The effects of the abnormal increasing of WBC on Hb value can be eliminated by the HiCN diluting blood,high-speed centrifuge to removing WBC and colorimetric assay.