• 中国科技论文统计源期刊
  • 中国科技核心期刊
  • 中国高校优秀期刊
  • 安徽省优秀科技期刊
LIU Dian-jun, LIU Hui-hui, SHEN Jian-ming, TIAN Shao-jiang, WANG Li-ping, LI Jun-feng. The effect of reduced glutathione on glomerular endothelial function in diabetic rats[J]. Journal of Bengbu Medical University, 2014, 39(11): 1464-1466.
Citation: LIU Dian-jun, LIU Hui-hui, SHEN Jian-ming, TIAN Shao-jiang, WANG Li-ping, LI Jun-feng. The effect of reduced glutathione on glomerular endothelial function in diabetic rats[J]. Journal of Bengbu Medical University, 2014, 39(11): 1464-1466.

The effect of reduced glutathione on glomerular endothelial function in diabetic rats

More Information
  • Received Date: November 17, 2013
  • Objective: To observe the feature of glomerular endothelial function in rats with early diabetic nephropathy(DN),and evaluate the effect of reduced glutathione on glomerular endothelial function in diabetic rats.Methods: Streptozotocin(60mg/ kg) was injected into peritoneal cavity in the rats to establish animal DN model.The diabetic rats were randomly divided into DN model group and reduced glutathione treated group.Coherent kits were used to detect glomerular nitrogen monoxide(NO),the activity of glomerular endothelial nitric oxide synthase(eNOS),von Willebrand factor(vWF),urinary albumin(UA),superoxide dismutase(SOD),malondialdehyde(MDA) and glutathione peroxidase.Results: Compared with normal control group,glomerular NO,activities of glomerular eNOS were significantly decreased in early DN rats,while the level of vWF and UA significantly increased(P<0.01),and the level of UA was correlated with the level of glomerular vWF(P<0.05).Glomerular SOD,glutathione peroxidase significantly decreased in early DN rats,while MDA was significantly increased(P<0.01).Compared with DN model group,glomerular NO,activities of glomerular eNOS were significantly increased in early DN rats treated with reduced glutathione,while the level of vWF and UA were significantly decreased,and glomerular MDA was significantly increased in early DN rats treated with reduced glutathione(P<0.01).Conclusions: Glomerular endothelial function is indiscriminate in early DN rats,while the level of oxidative stress significantly increases.Reduced glutathione improves glomerular endothelial dysfunction in early DN rats,maybe through reducing oxidative stress.
  • [1]
    Schmieder RE.Endothelial dysfunction:how can one intervene at the beginning of the cardiovascular continuum?[J].J Hypertens Suppl,2006,24(2):S31-S35.
    [2]
    胡欣,张弘,郝丽莉,等.还原型谷胱甘肽对2型糖尿病内皮功能的影响[J].中国误诊学杂志,2008,8(25):6108-6109.
    [3]
    Tesch GH,Lim AK.Recent insights into diabetic renal injury from the db/db mouse model of type 2 diabetic nephropathy[J].Am J Physiol Renal Physiol,2011,300(2):F301-F310.
    [4]
    Bourque SL,Davidge ST,Adams MA.The interaction between endothelin-1 and nitric oxide in the vasculature:new perspectives[J].Am J Physiol Regul Integr Comp Physiol,2011,300(6):R1288-R1295.
    [5]
    Crabtree MJ,Hale AB,Channon KM.Dihydrofolate reductase protects endothelial nitric oxide synthase from uncoupling in tetrahydrobiopterin deficiency[J].Free Radic Biol Med,2011,50(11):1639-1646.
    [6]
    Adel A,Abdel-Salam Z,Nammas W.Low-Dose statin therapy improves endothelial function in type 2 diabetic patients with normal serum total cholesterol:a randomized placebo-controlled study[J].J Clin Hypertens,2010,12(10):820-825.
    [7]
    Dendorfer A,Dominiak P,Schunkert H.ACE inhibitors and angiotensin Ⅱ receptor antagonists[J].Handb Exp Pharmacol,2005,170(1):407-442.
    [8]
    Lu X,Guo X,Karathanasis SK,et al.Rosiglitazone reverses endothelial dysfunction but not remodeling of femoral artery in Zucker diabetic fatty rats[J].Cardiovasc Diabetol,2010,9(1):19-26.
    [9]
    Cheng ZJ,Gronholm T,Louhelainen M,et al.Vascular and renal effects of vasopeptidase inhibition and angiotensin-converting enzyme blockade in spontaneously diabetic Goto-Kakizaki rats[J].J Hypertens,2005,23(9):1757-1770.

Catalog

    Article views (2871) PDF downloads (35) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return