The effect of arsenic trioxide on apoptosis of melanoma B₁₆ cells

Objective: To study the effect of arsenic trioxide on apoptosis of melanoma B₁₆ cells.Methods: Early apoptosis rate,late apoptosis rate and total apoptosis rate in melanoma B₁₆ cells were analysed by flow cytometry.Results: At the doses of arsenic trioxide were 5 μmol/L,10 μmol/L,or 20 μmol/L,the early apoptosis rates in melanoma B₁₆ cells were 7.18%,21.1%,and 3.59%,respectively;the late apoptosis rates were 2.63%,5.35%,and 6.34%,respectively;the total apoptosis rates were 9.81%,26.45%,and 9.93%,respectively.Conclusions: The early apoptosis rate and the late apoptosis rate in melanoma B₁₆ cells have dose-dependent relationship with arsenic trioxide.