Effect of arsenic trioxide on induction of apoptosis of melanoma A₃₇₅ cells

Objective: To study the effect of arsenic trioxide on induction of apoptosis of melanoma A₃₇₅ cell line.Methods: Early apoptosis rate and late apoptosis rate in A₃₇₅ cells were analysed by flow cytometry.Results: After pretreated with arsenic trioxide of 5 μmol/L,10 μmol/L,and 20 μmol/L,the early apoptosis rate in A₃₇₅ cells were 9.75%,50.9%,and 43.7%,respectively.The late apoptosis rate in A₃₇₅ cells were 2.10%,4.61%,and 11.2%,respectively.The totle apoptosis rate were 11.85%,55.51%,and 54.9%,respectively.Conclusions: The early and the late apoptosis rate in A₃₇₅ cells were induced by arsenic trioxide.