Regulation of ENaC subunits by mTORC2/SGK1 signaling pathway in vitro

Objective To discuss the influence of mTORC2/SGK1 signaling pathways on ENaC subunits in vitro;

Methods The ALI model was successfully established through the cultivation of A549 cells. The expression levels of inflammatory mediators, ENaC subunits, mTORC, p-Nedd4-2 and SGK1 (p-SGK1） were detected by ELISA, cell immunofluorescence and western blotting.

Results Compared with control group, the expressions of ENaC-α, ENaC-β, ENaC-γ in LPS group were decreased (P ＜ 0.05）. The differences were statistically significant. IL-6, IL-8, TNF-α in LPS group increased compared with those in control group and the differences were statistically significant (P ＜ 0.05）. Compared with the control group, the expression of mTORC was increased and the expressions of SGK1 (p-SGK1） and p-Nedd4-2 were decreased in LPS group (P ＜ 0.05）.

Conclusions mTORC2/SGK1 signaling pathway can regulate the expression of ENaC, and inflammatory mediators play an essential role in the occurrence and development of acute lung injury.