A comparative study of dried blood spot genomic DNA isolation kit and boiling method in DNA extraction and PCR detection of Plasmodium vivax

Objective: To compare the differences of dried blood spot(DBS) genomic DNA isolation kit and the boiling method in DNA extraction,PCR and cloning of Plasmodium vivax. Methods: The peripheral blood was collected from vivax malaria patients and the dried blood spots were prepared. The genomic DNA of ten samples was extracted with boiling method and QIAamp DNA mini kit (QIAGEN,Germany). The lactate dehydrogenase(LDH) gene was amplified by using the extracted DNA as template. The amplified fragment was purified and cloned into pGEM vector. The differences of both methods were analyzed and compared. Results: The target fragment of LDH gene was amplified with the gDNA extracted by the both methods; but the electrophoretic bands with the template by kit method were obviously brighter than that by boiling method. Conclusions: The boiling method is a simple, rapid and economical way in extracting gDNA from the dried blood spots; but in a given amount of blood sample, the extraction rate and purity of DNA are lower; while the extraction kit, with a higher extraction rate, is less affected by in the quantitative analysis or multiplex PCR.