Cloning and sequential analysis of ESAT6 gene of <i>Mycobacterium tuberculosis</i> H37Ra

WANG Ying; XUE Yu-qin; WANG Xue-mei; CHEN Yong; LI Jiang-yan; LI Qian; TANG Jie; XIA Hui; FANG Qiang

Journal of Bengbu Medical University > 2014 > 39(10): 1324-1326.

WANG Ying, XUE Yu-qin, WANG Xue-mei, CHEN Yong, LI Jiang-yan, LI Qian, TANG Jie, XIA Hui, FANG Qiang. Cloning and sequential analysis of ESAT6 gene of Mycobacterium tuberculosis H37Ra[J]. Journal of Bengbu Medical University, 2014, 39(10): 1324-1326.

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Cloning and sequential analysis of ESAT6 gene of Mycobacterium tuberculosis H37Ra

WANG Ying^1,2,
XUE Yu-qin^1,2,
WANG Xue-mei^1,2,
CHEN Yong^2,3,
LI Jiang-yan^1,2,
LI Qian^1,2,
TANG Jie^2,3,
XIA Hui^1,2,
FANG Qiang^1,2

1. Department of Microbiology and Parasitology, Bengbu Medical College, Bengbu Anhui 233030, China;
2. Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu Anhui 233030, China;
3. Department of Immunology, Bengbu Medical College, Bengbu Anhui 233030, China

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Received Date: March 19, 2013

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Objective: To clone and sequence the ESAT6 gene from Mycobacterium tuberculosis(MTB) H37Ra.Methods: The encoding gene of ESAT6 was amplified from MTB H37Ra genomic DNA using PCR technique.The amplified PCR product was subcloned into pTG19-T vector.The target gene was identified by PCR,single and double enzyme digestion,sequencing and sequence alignment analysis with BLAST software.Results: The ESAT6 gene was successfully cloned.The sequencing showed the fragment length was 288 bp,which was homology with the MTB H37Ra gene reported by GenBank.Conclusions: The coding gene of ESAT6 from MTB H37Ra is successfully cloned.
- Mycobacterium tuberculosis,
- H37Ra,
- ESAT6,
- clone,
- sequence analysis

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Cloning and sequential analysis of ESAT6 gene of Mycobacterium tuberculosis H37Ra

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