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    WU Xiao-li, ZHANG Lin-jie, XU Jian. Constructing of interleukin-18 eukaryotic expression vector and expression of interleukin-18 in HO-8910 cell line[J]. Journal of Bengbu Medical University, 2008, 33(2): 144-146.
    Citation: WU Xiao-li, ZHANG Lin-jie, XU Jian. Constructing of interleukin-18 eukaryotic expression vector and expression of interleukin-18 in HO-8910 cell line[J]. Journal of Bengbu Medical University, 2008, 33(2): 144-146.
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    Constructing of interleukin-18 eukaryotic expression vector and expression of interleukin-18 in HO-8910 cell line

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    • Objective: To constrct the eukaryotic expression vector of interleukin-18(IL-18) and study its expression in HO-8910 cell line.Methods: Interleukin-18 gene was inserted into the eukaryotic expression vector pCI with molecular cloning technique,transferred to HO-8910 cell line with Lipotectamine.Three transferred groups were studied:HO-8910+Lipotectamine,HO-8910+pCI and HO-8910 + pCI-hIL-18.The expression of IL-18 gene was comfirmed by RT-PCR technique.Results: The eukaryotic expression vector of IL-18(pCI-hIL-18) was successfully constructed and transferred to HO-8910 cells and expressed in mRNA and protein level of the HO-8910 cell line,but not expressed in the other two groups.Conclusions: The IL-18 gene could be expressed in the HO-8910 cell line.
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