Experimental study on determination of viability of the adult of Trichinella spiralis in vitro

Objective: To explore the prompt methods in determination of viability of the adult of Trichinella spiralis. Methods: The adult worms of Trichinella spiralis were stained with 0.5% safranine,1.0% neutral red or 0.5% methylene blue. Results: The dead adults were observed when stained with 0.5% safranine or 1.0% neutral red in the study. The vital adults were unstained with all three stains. Conclusions: Both 0.5% safranine staining and 1.0% neutral red staining are prompt method in determination of viability of the adult of Trichinella spiralis.