Real-time fluorescence quantitative PCR in determination of hepatitis B virus DNA

Objective: To explore the role of the real time fluorescence quantitative polymerase chain reaction (FQ PCR) in detecting heptitis B virus (HBV) DNA.Methods: The sera from 196 patients were measured by FQ PCR.Results: In the 112 HBeAg (+)/HBeAb (-) samples,the FQ PCR results were all positive,with 1.08×10⁷/ml of HBV DNA on the average.In the 68 HBeAg (-)/HBeAb (+) samples,the positive rate was 66.2% with a duplication amount of 6.12×10⁵/ml on the average.In the 16 HBeAg (-)/HBeAb (-) samples,the duplication amount was 2.72×10⁴/ml.Conclusions: FQ PCR can ensure the accurate quantity.It is a useful method in understanding HBV duplication and evaluating the therapeutic effect.