Features of immunophenotype of acute myeloid leukemia and its clinical significance

Objective: To investigate the characteristics and clinical significance of immunophenotype in acute myeloid leukemia (AML). Methods: Immunophenotype was carried out in 145 patients with AML using flow cytometry. Results: The expression of MPO, CD13,HLA-DR,CD33 and CD34 was 93.1%,90.3%,77.2%,71.7% and 70.3%,respectively. CD14 was expressed only in the M4 and M5 subtype. The lymphoid antigen expression was most commonly observed in CD7 (34.5%),especially in M5 subtype and CD19 (10.3%); CD2 was expressed only in part of M3 subtype. Two cases of acute hybrid leukemia were detected,expressing both myeloid and lymphoid associated antigen. Conclusions: MPO, CD13 and CD33 are sensitive myeloid associated antigens. The expression of CD7,CD19 and CD2,as well as the immunophenotype by flow cytometry,is of great value in diagnosis and prognosis of AML.