Establishment and characterization of mice hybridoma cell line secreting monoclonal antibody against cysticercus cellulosae KD26 antigen

Objective: To develop and identify the monoclonal antibody(McAb) against cysticercus cellulosae KD26 antigen,so as to provide useful help and further study.Methods: BALB/C mice were immunized 3 times with KD26 antigen of cysticercus cellulosae by SDS-PAGE(mixed with Freund's incomplet adjuvant),and the spleen cells of immunized mice were fused with myeloma cell line SP2/0(in the ratio of 10:1) in the HAT medium containing 50% PEG(4 000 MW),then incubated,selected and cloned.Numerical analysis on chromosome was performed by using metephase technique.The type and titer of the McAb was assayed by agar double diffusion technique and ELISA methods.Results: After being selected and cloned 3 times by using routine limited dilution method,one hybridoma cell line XH was generated,which contained 96 chromosomes and could steadily secrete specific McAb.The McAb belonged to IgG1 type.The titer was 1:1 000.The McAb had no cross reactions with the antigens of Schistosome japonicum,Toxoplasma gondii and Echinococcus granulosus.Conclusions: By the hybridoma technique,we have established the hybridoma cell line which can secrete specific and high titers McAb against cysticercus cellulosae KD26 antigen.