Construction for fluorescent eukaryotic cell expression vector of small proline-rich repeat protein 1A gene

Objective: To construct fluorescent eukaryotic expression vector of small proline-rich repeat protein 1A(SPRR1A) gene.Methods: The coding sequence of SPRR1A was amplified by polymerase chain reaction,the SPRR1A gene was cloned into plasmid pEGFP-N1,and the recombinant vector was selected and identified by restriction enzyme analysis and nucleotide sequence determination.Results: Correct construction of pEGFP-N1-SPRR1A was identified by methods of restriction enzyme analysis and nucleotide sequence determination.Conclusions: Eukaryotic expression plasmid pEGFP-N1-SPRR1A has been successfully constructed.