Effect of 5-Fu combined with recombinant human growth hormone (rhGH) on renal carcinoma cell line

Objective: To study the effect of rhGH on renal carcinoma cells line, so as to improve the treatment results.Methods: GRC-1 cells were cultured respectively for 24 hour in the control group, the rhGH group, the 5-Fu group and the rhGH +5-Fu group.The number of different phase cells was determined by flow cytometry (FCM).Results: The cells in G₀ to G₁ phase and S phase accounted for 53.4% and 42.1% respectively in the control group, but 41.1% and 52.7% in the rhGH group.The difference was significant(P<0.01 and P<0.05).In the 5-Fu group, the number of cells increased in G₀ to G₁ phase(62.3%), while it decreased in S phase (37.5%).In the rhGH+5-Fu group, the number of cells increased further in G₀ to G₁ phase(67.4%), while it decreased further in S phase (32.5%).There was no obvious difference in the two groups (P>0.05).Conclusions: rhGH may induce differentiation of GRC-1 cells but can not strengthen Fluorouraci in suppressing GRC-1 cells.